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| Registros recuperados: 37 | |
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| Ooka, Hideo; Terkawi, Mohamad A; Cao, Shinuo; Aboge, Gabriel; Goo, Youn-Kyoung; Luo, Yuzi; Li, Yan; Nishikawa, Yoshifumi; Igarashi, Ikuo; Xuan, Xuenan; 西川, 義文; 五十嵐, 郁男; 玄, 学南. |
| A cDNA encoding the Babesia microti 32-kDa protein was identified by serological immunoscreening of a cDNA expression library and designated as BmP32. The full length of BmP32 contains an open reading frame of 918 base pairs consisting of 306 amino acids having a significant homology with B. microti secreted antigen 1. Antiserum raised against recombinant protein (rBmP32) specifically reacted with a 32-kDa native protein of the parasite lysate using western blot analysis. The indirect immunofluorescent antibody test showed a preferable localization of BmP32 in the cytoplasm of the intra- and extracellular parasites. Moreover, BmP32 was secreted in the cytosol of infected erythrocytes, especially during the peak parasitemia and the recovery phase of the... |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3820 |
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| CAO, Shinuo; ZHANG, Shoufa; JIA, Lijun; XUE, Shujiang; YU, Longzheng; KAMYINGKIRD, Ketsarin; MOUMOUNI, Paul Franck Adjou; MOUSSA, Ahmed Abd El Moniem; ZHOU, Mo; ZHANG, Yuanming; TERKAWI, Mohamad Alaa; MASATANI1), Tatsunori; NISHIKAWA, Yoshifumi; XUAN, Xuenan; 西川, 義文; 玄, 学南. |
| Ovine theileriosis is a tick-borne disease that restricts the development of small ruminant husbandry in northern China. In this study, we report on a molecular epidemiological survey of ovine Theileria spp. in 198 blood samples taken from sheep in northern China. The DNA samples were screened by a nested polymerase chain reaction (PCR) targeting the 18S rRNA gene of ovine Theileria spp. The prevalence of ovine Theileria spp. in Yanji, Nongan, Longjing, Toudao and Jinchang was 80%, 40%, 37%, 24% and 32%, respectively. The sequencing analyses approved the present of the T. orientalis and/or T. luwenshuni in these regions. Taken together, we have demonstrated a high incidence of Theileria spp. in northern China that calls for the need to design effective... |
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Palavras-chave: China; Ovine Theileria spp; Sheep; Theileria luwenshuni. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3924 |
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| KHUKHUU, Altangerel; LAN, Dinh Thi Bich; LONG, Phung Thang; UENO, Akio; LI, Yan; LUO, Yuzi; MACEDO, Alan Caine Costa de; MATSUMOTO, Kotaro; INOKUMA, Hisashi; KAWAZU, Shin-Ichiro; IGARASHI, Ikuo; XUAN, Xuenan; YOKOYAMA, Naoaki; 松本, 高太郎; 猪熊, 壽; 河津, 信一郎; 五十嵐, 郁男; 玄, 学南; 横山, 直明. |
| Theileria orientalis is a benign bovine protozoan parasite that occasionally causes serious economic loss in the livestock industry. We report the findings of a molecular epidemiological survey of T. orientalis in 94 Vietnamese yellow cattle, 43 water buffaloes, 21 sheep, 21 goats and 85 blood-sucking ticks of cattle in the Thua Thien Hue province of Vietnam. The major piroplasm surface protein (MPSP) gene of T. orientalis was detected using polymerase chain reaction from 13 cattle (13.8%), 11 water buffaloes (25.6%), 1 sheep (4.8%) and 9 ticks (10.6%). Phylogenetic analysis using MPSP gene sequences showed the presence of seven genotypes, four previously categorized genotypes (Types 1, 3, 5 and 7) and three new genotypes (Types N-1, N-2 and N-3). |
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Palavras-chave: Cattle; Genotype; MPSP; Theileria orientalis; Vietnam. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3585 |
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| Altangerel, Khukhuu; Sivakumar, Thillaiampalam; Inpankaew, Tawin; Jittapalapong, Sathaporn; Terkawi, Mohamad Alaa; Ueno, Akio; Xuan, Xuenan; Igarashi, Ikuo; Yokoyama, Naoaki; 玄, 学南; 五十嵐, 郁男; 横山, 直明. |
| Here we report on an epidemiological study regarding the molecular prevalence of different genotypes of Theileria orientalis present among domestic cattle and water buffalo populations bred in Thailand. A phylogenetic analysis based on the parasitic gene encoding a major piroplasm surface protein revealed the presence of 5 genotypes (Types 1, 3, 5, 7, and N-3) in cattle and 7 genotypes (Types 1, 3, 4, 5, 7, N-2, and N-3) in water buffaloes. Types 4, 7, and N-3 of T. orientalis were reported for the first time in water buffaloes. The previously reported C and Thai types from Thailand clustered as types 7 and 6, respectively, in the present analysis. Great similarities were observed among nucleotide sequences of isolates of the same genotype from cattle and... |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3592 |
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| Seng, Seyha; Maki, Yoshiyuki; Yokoyama, Minesuke; Suzuki, R.; Kato, Mihoko; Bray, R. L.; Lim, C.; Zayatiin, Batsukh; Kamada, Takenori; Inoue, Noboru; Xuan, Xuenan; Igarashi, Ikuo; Nagasawa, Hideyuki; Fujisaki, Kozo; Mikami, Takeshi; Suzuki, Naoyoshi; Toyoda, Yutaka; 井上, 昇; 玄, 学南. |
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Palavras-chave: SAG-1; Oral wash and two-step polymerase chain reaction. |
| Ano: 1999 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/311 |
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| SIVAKUMAR, Thillaiampalam; LAN, Dinh Thi Bich; LONG, Phung Thang; YOSHINARI, Takeshi; TATTIYAPONG, Muncharee; GUSWANTO, Azirwan; OKUBO, Kazuhiro; IGARASHI, Ikuo; INOUE, Noboru; XUAN, Xuenan; YOKOYAMA, Naoaki; 井上, 昇; 玄, 学南; 横山, 直明. |
| Hemoprotozoan infections often cause serious production losses in livestock. In the present study, we conducted a PCR-based survey of Babesia bovis, Babesia bigemina, Theileria annulata, Theileria orientalis, Trypanosoma evansi and Trypanosoma theileri, using 423 DNA samples extracted from blood samples of cattle (n=202), water buffaloes (n=43), sheep (n=51) and goats (n=127) bred in the Hue and Hanoi provinces of Vietnam. With the exception of T. annulata and T. evansi, all other parasite species (B. bovis, B. bigemina, T. orientalis and T. theileri) were detected in the cattle populations with B. bovis being the most common among them. Additionally, four water buffaloes and a single goat were infected with B. bovis and B. bigemina, respectively. The Hue... |
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Palavras-chave: Epidemiology; Hemoprotozoa; Livestock; PCR; Vietnam. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4015 |
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| Sivakumar, T; Khukhuu, A; Igarashi, I; Xuan, X; Guo, G; Huang, X; Yokoyama, N; 五十嵐, 郁男; 玄, 学南; 横山, 直明. |
| A molecular epidemiological survey on Theileria orientalis was conducted in a cattle population of Fujian province in China. The screening polymerase chain reaction assay showed that 23 (45%) of 51 blood samples were positive for T. orientalis. DNA sequencing of the major piroplasm surface antigen-encoding gene indicated the presence of 3 different genotypes in the study area(types 1，2，and 5) and identified T. orientalis types 1 and 2 as the major genotypes in the sample population. Because type 2 (Ikeda type in Japan) has been recognized as a relatively virulent genotype of T. orientalis，control and preventive measures to minimize the incidence of infectious diseases among cattle bred in China are necessary. |
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Palavras-chave: Theileria orientalis; Cattle; China; Epidemiology; Genotype. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3098 |
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| Chahan, Bayin; Jian, Zijian; Xuan, Xuenan; Sato, Yukita; Kabeya, Hidenori; Tuchiya, Kotaro; Itamoto, Kazuhito; Okuda, Masaru; Mikami, Takeshi; Maruyama, Soichi; Inokuma, Hisashi; 玄, 学南; 猪熊, 壽. |
| Serological methods were utilized to detect Anaplasma and Ehrlichia infection in domestic animals in Xinjiang Uygur Autonomous Region, China. By using an indirect immunofluorescence assay (IFA), antibodies that reacted with Anaplasma phagocytophilum and Ehrlichia chaffeensis were detected mainly in ruminants kept on pastureland in Altai, Ili and Kashgar area. Antibody titers up to 1:320 were recorded. These results indicate that ruminants kept in these areas may be infected with some species of Anaplasma and Ehrlichia. |
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Palavras-chave: Anaplasma; Ehrlichia domestic animals seroepidemiology Xinjiang Uygur Autonomous Region China. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/742 |
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| KAWASE, Osamu; GOO, Youn-Kyoung; JUJO, Hayato; NISHIKAWA, Yoshifumi; XUAN, Xuenan; 西川, 義文; 玄, 学南. |
| Saponin is the generic name of steroid or triterpene glycosides, and the capacities of some saponins to stimulate both Th1 immune response and production of cytotoxic T cells are useful as vaccine components against intracellular pathogens. Because saponins have been found commonly in starfish, we assessed the potential of starfish, Asterias amurensis and Asterina pectinifera, as adjuvant sources. Crude starfish saponins had hemolytic activities (EC50=10 to 100 μg/ml) and thin layer chromatography indicated heterogeneity of their constituents. When starfish saponis were subcutaneously injected into mice with ovalbumin (OVA), OVA-specific IgG, especially IgG2a instead of IgG1 was produced in mouse blood, suggesting starfish saponins stimulated Th1 type... |
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Palavras-chave: Adjuvant; Saponin; Starfish; Th1 immunity; Vaccine. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3587 |
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| Buates, S.; Xuan, Xuenan; Igarashi, Makoto; Sugimoto, Chihiro; Inoue, Noboru; 玄, 学南; 五十嵐, 慎; 井上, 昇. |
| Toxoplasma gondii microneme protein 2 (TgMIC2), an apically stored adhesin, was shown to be a key participant involved in the initial attachment and invasion to a host cell. In this study, we had established the clonal line of T. gondii tachyzoites which over-expressed TgMIC2 using the tetracycline repressor (TetR)-based inducible gene expression system. The TgMIC2 over-expression significantly reduced tachyzoite propagation in vitro (p < 0.002) and significantly reduced the parasite virulence in mice (p = 0.002). The over-expression of exogenous TgMIC2 caused the increase of mRNA expression level of endogenous TgMIC2. Wild type parasites mainly expressed TgMIC2115 protein, the microneme store and the surface form of TgMIC2. Over-expression of TgMIC2... |
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Palavras-chave: Toxoplasma gondii; TgMIC2; Over-expression. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2236 |
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| Fujii, Kei; Yokoyama, Naoaki; Takabatake, Noriyuki; Suzuki, Hiroshi; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 鈴木, 宏志; 玄, 学南; 五十嵐, 郁男. |
| In hemoprotozoa，gene transfer technology provides an important tool to aid in the functional study of parasite genes. In this study，a transfer vector containing the enhanced green fluorescent protein (EGFP) gene laid between the Toxoplasma gondii GRAI promoter region and the GRA2 polyA signal region was constructed and transfected into the in vitro culture of Babesia boνis by the electroporation method.On the first and second days post-transfection，clear positive fluorescences were detected in some parasite bodies， indicating the successful expression of the EGFP gene controlled by the GRA-1 promoter in B. bovis. However， the positive parasites were not ubiquitous and finally disappeared until the forth day post-transfection. This is the first time that... |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/631 |
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| 玄, 学南; XUAN, XUENAN. |
| 本研究では、プロテオミックス法によるネオスポラ原虫の全虫体タン パク質の網羅的解析を行った。MALDI-TOF-MS 解析により約600 種類の可視化されたタンパク質 の中から約60 種類のタンパク質の同定に成功した。そのうちの一部は原虫の宿主細胞への吸 着・侵入ならびにエネルギー代謝に関わるタンパク質であることが示唆された。これらの成果 は、ネオスポラ原虫の新規ワクチン・治療薬開発に繋がるものとして期待される。 The aims of this study were to widen our view on the global expression of protein profile of Neospora caninum parasite and to discover vaccine and drug target molecules for control of neosporosis. Approximately 600 protein spots were detected on 2-DE, and 60 proteins were identified by MALDI-TOF-MS analysis. Some of the identified proteins were predicted to be involved in the invasion of the parasite into the host cells and in parasite metabolism. Therefore, some of these proteins could be potential vaccine candidates and drug targets. |
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Palavras-chave: ネオスポラ; トキソプラズマ; プロテオミックス; ワクチン; 治療薬; MALDI-TOF-MS; 抗原; 酵素. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2759 |
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| 久保田, 直樹; 坂田, 義美; 宮崎, 直美; 板本, 和仁; 坂内, 天; 西川, 義文; 玄, 学南; 猪熊, 壽; NISHIKAWA, Yoshifumi; XUAN, Xuenan; INOKUMA, Hisashi. |
| 組換抗原を用いた種特異的ELISAにより,犬のNeospora caninum感染について全国的血清疫学調査を実施した.1,206頭中全国30県から126頭(10.4%)が陽性を示した.陽性率は,雄よりも雌で有意に高く,また他品種と比較して有意に高い陽性率を示す品種がみられた.子宮蓄膿症および糖尿病の病歴を有する犬の陽性率は,他の疾病を有する犬または疾病のない犬の陽性率に比べて高値であった. A seroepidemiological survey of Neospora caninum infection among dogs in Japan was conducted using species-specific enzyme-linked immunosorbent assay with recombinant surface antigen (Nc-SAG1t). Among 1,206 dogs examined, 126 dogs (10.4%) from 30 prefectures from Hokkaido to Okinawa were positive to N. caninum infections, which were more frequently detected in females than males. Siberian Huskies showed the highest positive rate compared with the other breeds. Dogs with pyometra and diabetes mellitus showed the higher positive rates than dogs with other... |
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Palavras-chave: Canine; Nbospora caninum; Species-specific ELISA. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4003 |
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| Registros recuperados: 37 | |
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